You'd have to compare the ROM images to other know images for matches. I don't think there is any sort of "odometer" or "broken seal" if that's what you mean.
If someone read out their stock ROM image, then did an APflash , then an Ecutek flash, then flashed back to the stock image that was read before any flashing, I don't think anyone could ever tell.
1. The ROM image does not match any stock image
2. The ROM image does not match what's on the case AND was not a likely dealer update image for what was on the case.
Thank you for all inputs. So Which one is the best to use in referencing each ECU? I guess calibration ID is better right? But will it be changed if I flash my ECU with ECUflash?